Composite
Part:BBa_K1072000:Design
Designed by: Yichong wang, Rui chen, Xihao liao and Junjie zhang Group: iGEM13_SCUT (2013-08-31)
Lux pL controlled LuxR + lux pR autoinducing LuxI (lva tag) + lux pR controlled GFP.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1821
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1101
Illegal BsaI.rc site found at 1970
Illegal BsaI.rc site found at 2697
Design Notes
Constructing device compositing oscillator, coupling, reporter and productor.This part include the oscillator and reporter.
Source
Get from kit plate
References
1. Dong YH, Zhang LH.Quorum sensing and quorum-quenching enzymes.J Microbiol. 2005 Feb;43 Spec No:101-9.
2. Danino T, Mondragón-Palomino O, Tsimring L, Hasty J. A synchronized quorum of genetic clocks. Nature. 2010 Jan 21;463(7279):326-30.
3. Prindle A, Samayoa P, Razinkov I, Danino T, Tsimring LS, Hasty J.a sensing array of radically coupled genetic biopixels. Nature. 2011 Dec 18;481(7379):39-44.